imaging mass cytometry Search Results


hyperion mass cytometry imaging system  (fluidigm)
93
fluidigm hyperion mass cytometry imaging system
a-b , Heterogeneity in T cell infiltration in chordomas. The images show T cell-related markers (CD4 = red, CD8 = blue), a proliferation marker (Ki-67 = yellow) and a tumour marker (keratin = white) as detected by imaging mass <t>cytometry.</t> a , chordoma with low T cell infiltration (ICR low). b , chordoma with high T cell infiltration and a magnified image of the chordoma with high T cell infiltration (ICR high). c , Heatmap showing the mean cell density Z-Score of all T cell phenotypes in all chordoma samples ( n = 32), hierarchically clustered into a T cell high ( n = 18) and T cell low ( n = 14) group. Samples are annotated with their respective ICR score from the transcriptional analysis. In addition, the samples are annotated for their anatomical location, the follow-up status of the patients as well as whether the sample had received neoadjuvant radiotherapy. d , Boxplots presenting the mean cell densities per mm 2 per classified T cell group (T cell high: n = 18; T cell low: n = 14) for the identified T cell phenotypes. e , Kaplan-Meier curves interrogating the association between T cell infiltration and disease-specific survival. Two-sided log-rank tests were performed for the Kaplan Meier estimates. ICR = immunologic constant of rejection; Tregs = regulatory T cells
Hyperion Mass Cytometry Imaging System, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+mass+cytometry/bio_rxiv__2023__09__14__557579-162-15-20?v=fluidigm
Average 93 stars, based on 1 article reviews
hyperion mass cytometry imaging system - by Bioz Stars, 2026-08
93/100 stars
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imaging mass cytometry (imc)  (AstraZeneca ltd)
90
AstraZeneca ltd imaging mass cytometry (imc)
a-b , Heterogeneity in T cell infiltration in chordomas. The images show T cell-related markers (CD4 = red, CD8 = blue), a proliferation marker (Ki-67 = yellow) and a tumour marker (keratin = white) as detected by imaging mass <t>cytometry.</t> a , chordoma with low T cell infiltration (ICR low). b , chordoma with high T cell infiltration and a magnified image of the chordoma with high T cell infiltration (ICR high). c , Heatmap showing the mean cell density Z-Score of all T cell phenotypes in all chordoma samples ( n = 32), hierarchically clustered into a T cell high ( n = 18) and T cell low ( n = 14) group. Samples are annotated with their respective ICR score from the transcriptional analysis. In addition, the samples are annotated for their anatomical location, the follow-up status of the patients as well as whether the sample had received neoadjuvant radiotherapy. d , Boxplots presenting the mean cell densities per mm 2 per classified T cell group (T cell high: n = 18; T cell low: n = 14) for the identified T cell phenotypes. e , Kaplan-Meier curves interrogating the association between T cell infiltration and disease-specific survival. Two-sided log-rank tests were performed for the Kaplan Meier estimates. ICR = immunologic constant of rejection; Tregs = regulatory T cells
Imaging Mass Cytometry (Imc), supplied by AstraZeneca ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+mass+cytometry/pm40452454-3590-22-0?v=AstraZeneca+ltd
Average 90 stars, based on 1 article reviews
imaging mass cytometry (imc) - by Bioz Stars, 2026-08
90/100 stars
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multi plexed tissue imaging  (Spatial Transcriptomics Inc)
86
Spatial Transcriptomics Inc multi plexed tissue imaging
a-b , Heterogeneity in T cell infiltration in chordomas. The images show T cell-related markers (CD4 = red, CD8 = blue), a proliferation marker (Ki-67 = yellow) and a tumour marker (keratin = white) as detected by imaging mass <t>cytometry.</t> a , chordoma with low T cell infiltration (ICR low). b , chordoma with high T cell infiltration and a magnified image of the chordoma with high T cell infiltration (ICR high). c , Heatmap showing the mean cell density Z-Score of all T cell phenotypes in all chordoma samples ( n = 32), hierarchically clustered into a T cell high ( n = 18) and T cell low ( n = 14) group. Samples are annotated with their respective ICR score from the transcriptional analysis. In addition, the samples are annotated for their anatomical location, the follow-up status of the patients as well as whether the sample had received neoadjuvant radiotherapy. d , Boxplots presenting the mean cell densities per mm 2 per classified T cell group (T cell high: n = 18; T cell low: n = 14) for the identified T cell phenotypes. e , Kaplan-Meier curves interrogating the association between T cell infiltration and disease-specific survival. Two-sided log-rank tests were performed for the Kaplan Meier estimates. ICR = immunologic constant of rejection; Tregs = regulatory T cells
Multi Plexed Tissue Imaging, supplied by Spatial Transcriptomics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+mass+cytometry/pm41015223-57-18-13?v=Spatial+Transcriptomics+Inc
Average 86 stars, based on 1 article reviews
multi plexed tissue imaging - by Bioz Stars, 2026-08
86/100 stars
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image mass cytometry imc analyses  (Vectra Laboratories)
86
Vectra Laboratories image mass cytometry imc analyses
a-b , Heterogeneity in T cell infiltration in chordomas. The images show T cell-related markers (CD4 = red, CD8 = blue), a proliferation marker (Ki-67 = yellow) and a tumour marker (keratin = white) as detected by imaging mass <t>cytometry.</t> a , chordoma with low T cell infiltration (ICR low). b , chordoma with high T cell infiltration and a magnified image of the chordoma with high T cell infiltration (ICR high). c , Heatmap showing the mean cell density Z-Score of all T cell phenotypes in all chordoma samples ( n = 32), hierarchically clustered into a T cell high ( n = 18) and T cell low ( n = 14) group. Samples are annotated with their respective ICR score from the transcriptional analysis. In addition, the samples are annotated for their anatomical location, the follow-up status of the patients as well as whether the sample had received neoadjuvant radiotherapy. d , Boxplots presenting the mean cell densities per mm 2 per classified T cell group (T cell high: n = 18; T cell low: n = 14) for the identified T cell phenotypes. e , Kaplan-Meier curves interrogating the association between T cell infiltration and disease-specific survival. Two-sided log-rank tests were performed for the Kaplan Meier estimates. ICR = immunologic constant of rejection; Tregs = regulatory T cells
Image Mass Cytometry Imc Analyses, supplied by Vectra Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+mass+cytometry/pmc13134962-2-6-0?v=Vectra+Laboratories
Average 86 stars, based on 1 article reviews
image mass cytometry imc analyses - by Bioz Stars, 2026-08
86/100 stars
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Image Search Results


a-b , Heterogeneity in T cell infiltration in chordomas. The images show T cell-related markers (CD4 = red, CD8 = blue), a proliferation marker (Ki-67 = yellow) and a tumour marker (keratin = white) as detected by imaging mass cytometry. a , chordoma with low T cell infiltration (ICR low). b , chordoma with high T cell infiltration and a magnified image of the chordoma with high T cell infiltration (ICR high). c , Heatmap showing the mean cell density Z-Score of all T cell phenotypes in all chordoma samples ( n = 32), hierarchically clustered into a T cell high ( n = 18) and T cell low ( n = 14) group. Samples are annotated with their respective ICR score from the transcriptional analysis. In addition, the samples are annotated for their anatomical location, the follow-up status of the patients as well as whether the sample had received neoadjuvant radiotherapy. d , Boxplots presenting the mean cell densities per mm 2 per classified T cell group (T cell high: n = 18; T cell low: n = 14) for the identified T cell phenotypes. e , Kaplan-Meier curves interrogating the association between T cell infiltration and disease-specific survival. Two-sided log-rank tests were performed for the Kaplan Meier estimates. ICR = immunologic constant of rejection; Tregs = regulatory T cells

Journal: bioRxiv

Article Title: Multimodal profiling of chordoma immunity reveals distinct immune contextures

doi: 10.1101/2023.09.14.557579

Figure Lengend Snippet: a-b , Heterogeneity in T cell infiltration in chordomas. The images show T cell-related markers (CD4 = red, CD8 = blue), a proliferation marker (Ki-67 = yellow) and a tumour marker (keratin = white) as detected by imaging mass cytometry. a , chordoma with low T cell infiltration (ICR low). b , chordoma with high T cell infiltration and a magnified image of the chordoma with high T cell infiltration (ICR high). c , Heatmap showing the mean cell density Z-Score of all T cell phenotypes in all chordoma samples ( n = 32), hierarchically clustered into a T cell high ( n = 18) and T cell low ( n = 14) group. Samples are annotated with their respective ICR score from the transcriptional analysis. In addition, the samples are annotated for their anatomical location, the follow-up status of the patients as well as whether the sample had received neoadjuvant radiotherapy. d , Boxplots presenting the mean cell densities per mm 2 per classified T cell group (T cell high: n = 18; T cell low: n = 14) for the identified T cell phenotypes. e , Kaplan-Meier curves interrogating the association between T cell infiltration and disease-specific survival. Two-sided log-rank tests were performed for the Kaplan Meier estimates. ICR = immunologic constant of rejection; Tregs = regulatory T cells

Article Snippet: ROIs of 1000×1000 μm were selected with consecutive H&E slides and ablated by using a Hyperion mass cytometry imaging system (Fluidigm) at the Flow Core Facility (LUMC, Leiden) within a month after staining.

Techniques: Marker, Imaging, Mass Cytometry

a , Boxplots comparing cell densities between the T cell high ( n = 18) and T cell low groups ( n = 14). Dendritic cells (CD11c +/- HLA-DR + CD68 - ) and immune cell aggregates (CD45 + and multiple differentiation markers, e.g. CD3 + CD68 + ) are significantly higher in the T cell high group as compared to the T cell low group. Two-sided t-test was performed in R and the P value was adjusted with the Benjamini-Hochberg false discovery rate (FDR). * = P < 0.05; ** = P < 0.01; *** P < 0.001. b , An image generated with imaging mass cytometry displaying co-localisation of dendritic cells (HLA-DR = red) and T cells (CD3 = blue). Tumour cells are only shown in the overview image (top-left image, keratin = white). Other myeloid and T cell-related markers are presented in separate images around the overview image in green (CD4, CD8, CD11c, CD14 and CD163), to show co-localisation of different markers. c , Heatmap displaying the spatial interaction analysis results comparing the microenvironment of T cell high with T cell low chordomas. Only significant interactions are visualised and coloured for the subgroup(s) in which they are enriched. Permutation Z scores below 10 were considered not significant and thus removed for visualisation. Interactions of interest are indicated by black arrows. PDPN = podoplanin.

Journal: bioRxiv

Article Title: Multimodal profiling of chordoma immunity reveals distinct immune contextures

doi: 10.1101/2023.09.14.557579

Figure Lengend Snippet: a , Boxplots comparing cell densities between the T cell high ( n = 18) and T cell low groups ( n = 14). Dendritic cells (CD11c +/- HLA-DR + CD68 - ) and immune cell aggregates (CD45 + and multiple differentiation markers, e.g. CD3 + CD68 + ) are significantly higher in the T cell high group as compared to the T cell low group. Two-sided t-test was performed in R and the P value was adjusted with the Benjamini-Hochberg false discovery rate (FDR). * = P < 0.05; ** = P < 0.01; *** P < 0.001. b , An image generated with imaging mass cytometry displaying co-localisation of dendritic cells (HLA-DR = red) and T cells (CD3 = blue). Tumour cells are only shown in the overview image (top-left image, keratin = white). Other myeloid and T cell-related markers are presented in separate images around the overview image in green (CD4, CD8, CD11c, CD14 and CD163), to show co-localisation of different markers. c , Heatmap displaying the spatial interaction analysis results comparing the microenvironment of T cell high with T cell low chordomas. Only significant interactions are visualised and coloured for the subgroup(s) in which they are enriched. Permutation Z scores below 10 were considered not significant and thus removed for visualisation. Interactions of interest are indicated by black arrows. PDPN = podoplanin.

Article Snippet: ROIs of 1000×1000 μm were selected with consecutive H&E slides and ablated by using a Hyperion mass cytometry imaging system (Fluidigm) at the Flow Core Facility (LUMC, Leiden) within a month after staining.

Techniques: Generated, Imaging, Mass Cytometry

a, Boxplots with the significantly different phenotypes between the T cell high and T cell low groups. Two-sided t-test was performed in R and the P value was adjusted with the Benjamini-Hochberg false discovery rate (FDR). All presented phenotypes were significantly different between the T cell high an T cell low group. * = P < 0.05; ** = P < 0.01; *** P < 0.001. b , Representative images generated with imaging mass cytometry displaying the differentially abundant cell phenotypes in both T cell low and T cell high samples. Images are grouped per row for the displayed cell markers and outlined with their respective T cell group (T cell high = red rectangle & left, T cell low = blue rectangle & right). The top row presents CD11c + macrophages (CD11c = red, CD68 = green, HLA-DR = blue, keratin = white), the middle row displays granulocytes and CD163 + HLA-DR+ macrophages (CD15 = green, CD163 = blue, HLA-DR = red, keratin = white) and the bottom row shows vessels and podoplanin + stromal cells (CD31 = red, PDPN = green, keratin = white). c , Boxplots presenting the cumulative mean cell density per mm 2 of all immune cells, stromal cells and tumour cells in both T cell high and T cell low groups. Two-sided t-test was performed in R. ** = P < 0.01, *** = P < 0.001. PDPN = podoplanin.

Journal: bioRxiv

Article Title: Multimodal profiling of chordoma immunity reveals distinct immune contextures

doi: 10.1101/2023.09.14.557579

Figure Lengend Snippet: a, Boxplots with the significantly different phenotypes between the T cell high and T cell low groups. Two-sided t-test was performed in R and the P value was adjusted with the Benjamini-Hochberg false discovery rate (FDR). All presented phenotypes were significantly different between the T cell high an T cell low group. * = P < 0.05; ** = P < 0.01; *** P < 0.001. b , Representative images generated with imaging mass cytometry displaying the differentially abundant cell phenotypes in both T cell low and T cell high samples. Images are grouped per row for the displayed cell markers and outlined with their respective T cell group (T cell high = red rectangle & left, T cell low = blue rectangle & right). The top row presents CD11c + macrophages (CD11c = red, CD68 = green, HLA-DR = blue, keratin = white), the middle row displays granulocytes and CD163 + HLA-DR+ macrophages (CD15 = green, CD163 = blue, HLA-DR = red, keratin = white) and the bottom row shows vessels and podoplanin + stromal cells (CD31 = red, PDPN = green, keratin = white). c , Boxplots presenting the cumulative mean cell density per mm 2 of all immune cells, stromal cells and tumour cells in both T cell high and T cell low groups. Two-sided t-test was performed in R. ** = P < 0.01, *** = P < 0.001. PDPN = podoplanin.

Article Snippet: ROIs of 1000×1000 μm were selected with consecutive H&E slides and ablated by using a Hyperion mass cytometry imaging system (Fluidigm) at the Flow Core Facility (LUMC, Leiden) within a month after staining.

Techniques: Generated, Imaging, Mass Cytometry

a , Examples images of Human Leukocyte Antigen (HLA) class I (HCA2 and HC10) expression in a T cell high chordoma as validated by immunohistochemistry. b , A n example image and a corresponding magnitication of the same tissue core as displayed in ( a ) as generated by imaging mass cytometry. The images present intratumoral γδ T cell intiltration as indicated by the magenta arrows (CD3 = red, TCRγδ = blue, keratin = white). TCR = T cell receptor.

Journal: bioRxiv

Article Title: Multimodal profiling of chordoma immunity reveals distinct immune contextures

doi: 10.1101/2023.09.14.557579

Figure Lengend Snippet: a , Examples images of Human Leukocyte Antigen (HLA) class I (HCA2 and HC10) expression in a T cell high chordoma as validated by immunohistochemistry. b , A n example image and a corresponding magnitication of the same tissue core as displayed in ( a ) as generated by imaging mass cytometry. The images present intratumoral γδ T cell intiltration as indicated by the magenta arrows (CD3 = red, TCRγδ = blue, keratin = white). TCR = T cell receptor.

Article Snippet: ROIs of 1000×1000 μm were selected with consecutive H&E slides and ablated by using a Hyperion mass cytometry imaging system (Fluidigm) at the Flow Core Facility (LUMC, Leiden) within a month after staining.

Techniques: Expressing, Immunohistochemistry, Generated, Imaging, Mass Cytometry