imaging mass cytometry Search Results


imaging mass cytometry  (fluidigm)
98
fluidigm imaging mass cytometry
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Imaging Mass Cytometry, supplied by fluidigm, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging mass cytometry platform  (Inserm Transfert)
90
Inserm Transfert imaging mass cytometry platform
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Imaging Mass Cytometry Platform, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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desorption electrospray ionization imaging mass spectrometry of lipids in rat spinal cord  (Purdue University Cytometry)
90
Purdue University Cytometry desorption electrospray ionization imaging mass spectrometry of lipids in rat spinal cord
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Desorption Electrospray Ionization Imaging Mass Spectrometry Of Lipids In Rat Spinal Cord, supplied by Purdue University Cytometry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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image mass cytometry (imc)  (Vectra Laboratories)
90
Vectra Laboratories image mass cytometry (imc)
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Image Mass Cytometry (Imc), supplied by Vectra Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging mass cytometry  (Inserm Transfert)
90
Inserm Transfert imaging mass cytometry
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Imaging Mass Cytometry, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging mass cytometry  (Matisse Pharmaceuticals)
90
Matisse Pharmaceuticals imaging mass cytometry
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Imaging Mass Cytometry, supplied by Matisse Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(imaging mass cytometry microscopy single-cell segmentation)  (Matisse Pharmaceuticals)
90
Matisse Pharmaceuticals (imaging mass cytometry microscopy single-cell segmentation)
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
(Imaging Mass Cytometry Microscopy Single Cell Segmentation), supplied by Matisse Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging mass cytometry  (Nature Biotechnology)
90
Nature Biotechnology imaging mass cytometry
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Imaging Mass Cytometry, supplied by Nature Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging mass cytometry  (Biomedical Technologies)
90
Biomedical Technologies imaging mass cytometry
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Imaging Mass Cytometry, supplied by Biomedical Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging mass cytometry (imc)  (AstraZeneca ltd)
90
AstraZeneca ltd imaging mass cytometry (imc)
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Imaging Mass Cytometry (Imc), supplied by AstraZeneca ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging mass cytometry  (Bruker Corporation)
90
Bruker Corporation imaging mass cytometry
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Imaging Mass Cytometry, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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liad/apci mass spectra and images of the setup and titanium foil surfaces pdf  (Purdue University Cytometry)
90
Purdue University Cytometry liad/apci mass spectra and images of the setup and titanium foil surfaces pdf
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Liad/Apci Mass Spectra And Images Of The Setup And Titanium Foil Surfaces Pdf, supplied by Purdue University Cytometry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass cytometry. Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.

Journal: Science translational medicine

Article Title: Nonlesional lupus skin contributes to inflammatory education of myeloid cells and primes for cutaneous inflammation.

doi: 10.1126/scitranslmed.abn2263

Figure Lengend Snippet: Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass cytometry. Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.

Article Snippet: Imaging mass cytometry of tissue sections Formalin-fixed, paraffin-embedded skin biopsy tissue sections from lesional skin of patients with SCLE or DLE were analyzed using a Hyperion imaging cytometry by time of flight system (CyTOF) (Fluidigm) as previously described (34) with modifications of the antibody panel.

Techniques: Control, Staining, Expressing, Generated, Imaging, Mass Cytometry